SPIRE1 - Antibodies - The Human Protein Atlas

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SPIRE1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA040737

Antibody HPA040942

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA040737

HPA040942

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA040737

Antibody HPA040942

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA040737

Antibody HPA040942

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.

Antibody dilution

1:200

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA040737

Antibody HPA040942

Standard validation

Uncertain

Figure description

Immunohistochemical staining of human lateral ventricle shows strong nuclear positivity in neuronal cells.

Immunohistochemical staining of human liver shows strong cytoplasmic and membranous positivity in hepatocytes.

Expression

RNA: detected in 36 tissues
Protein: detected in 57 cell types

RNA: detected in 36 tissues
Protein: detected in 56 cell types

Retrieval

HIER pH6

Antibody dilution

1:1000

1:60

Literature conformity

Not consistent with gene/protein characterization data.

Consistent with gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA040737

Antibody HPA040942

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

85.5, 84, 71.6, 62.9

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA040737

Antibody HPA040942

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA040737

Antibody HPA040942

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

LEEIKAERKLRPVSPEEIRRSRLDVTTPESTKNLVESSMVNGGLTSQTKE
NGLSTSQQVPAQRKKLLRAPTLAELDSSESEEETLHK

LQRGESSMRSEKPSTAHHRPLRSIARFSSKSKSMDKSDEELQFPKELMED
WSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQ

Matching transcripts

SPIRE1-002 - ENSP00000387226 [100%]
SPIRE1-005 - ENSP00000309661 [100%]
SPIRE1-010 - ENSP00000407050 [100%]
SPIRE1-201 - ENSP00000486927 [100%]
SPIRE1-001 - ENSP00000387266 [86%]

SPIRE1-001 - ENSP00000387266 [100%]
SPIRE1-002 - ENSP00000387226 [100%]
SPIRE1-005 - ENSP00000309661 [100%]
SPIRE1-010 - ENSP00000407050 [100%]
SPIRE1-201 - ENSP00000486927 [100%]

Other gene match

ANTIGEN VIEW

SPIRE1-001	SPIRE1-002	SPIRE1-005	SPIRE1-010	SPIRE1-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.