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Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.
Antibody dilution
1:200
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA018127
Antibody HPA027887
Antibody CAB012302
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human parathyroid gland shows moderate cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 65 cell types
RNA: detected in 36 tissues Protein: detected in 61 cell types
RNA: detected in 36 tissues Protein: detected in 38 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:350
1:25
1:25
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA018127
Antibody HPA027887
Antibody CAB012302
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
202.5, 197.4, 160.8
202.5, 197.4, 160.8, 79.8
202.5, 197.4, 160.8, 79.8, 25.2, 24.6
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA018127
Antibody HPA027887
Antibody CAB012302
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
