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Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA056863 across 73 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA046864 across 73 cell types.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human esophagus shows moderate membranous positivity in squamous epithelial cells.
Immunohistochemical staining of human esophagus shows strong membranous and cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human esophagus shows moderate cytoplasmic and membranous positivity in squamous epithelial cells.
Expression
RNA: detected in 8 tissues Protein: detected in 5 cell types
RNA: detected in 8 tissues Protein: detected in 7 cell types
RNA: detected in 8 tissues Protein: detected in 38 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:650
1:600
1:250
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA046864
Antibody HPA056863
Antibody CAB069880
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
107.5
107.5
107.5
Antibody dilution
1:100
1:250
1:500
PROTEIN ARRAY
Antibody HPA046864
Antibody HPA056863
Antibody CAB069880
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.