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UGGT1

ANTIBODY INFORMATION
 
 

Antibody HPA012761

  

Antibody HPA015127

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA012761   HPA015127  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   4  

References

   1  

VALIDATION SUMMARY
 
 

Antibody HPA012761

  

Antibody HPA015127

  
Immunocytochemistry

  Uncertain  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA012761

  

Antibody HPA015127

  

Standard validation

   Uncertain  

   

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.  

Antibody dilution

   1:4  

Literature conformity

   The subcellular location is not consistent with literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA012761

  

Antibody HPA015127

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  

 

  
 

  

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA015127 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA012761 across 46 cell lines.		  
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 

  
 

  

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA015127 across 73 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA012761 across 73 cell types.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human epididymis shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human epididymis shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 68 cell types		   RNA: detected in 37 tissues
Protein: detected in 63 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:25  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA012761

  

Antibody HPA015127

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 177.2   177.2  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA012761

  

Antibody HPA015127

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA012761

  

Antibody HPA015127

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 126   139  

Antigen sequence

 
DFILSHAVYCRDVLKLKKGQRAVISNGRIIGPLEDSELFNQDDFHLLENI
ILKTSGQKIKSHIQQLRVEEDVASDLVMKVDALLSAQPKGDPRIEYQFFE
DRHSAIKLRPKEGETYFDVVAVVDPV
  
KVKVEHVVSVLEKKYPYVEVNSILGIDSAYDRNRKEARGYYEQTGVGPLP
VVLFNGMPFEREQLDPDELETITMHKILETTTFFQRAVYLGELPHDQDVV
EYIMNQPNVVPRINSRILTAERDYLDLTASNNFFVDDYA
 

Matching transcripts

 UGGT1-001 - ENSP00000259253 [100%]
  UGGT1-001 - ENSP00000259253 [100%]
 

Other gene match

    
ANTIGEN VIEW
UGGT1-001

RELEVANT PUBLICATIONS
 
 

Antibody HPA012761

  

Antibody HPA015127

  

1.

   Comparative Proteomics Reveals Important Viral-Host Interactions in HCV-Infected Human Liver Cells
Liu S et al
PLoS One 2016;11(1):e0147991
Application: IP, WB 		 
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.