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TET1

ANTIBODY INFORMATION
 
 

Antibody HPA019032

  

Antibody HPA057273

  

Antibody CAB014886

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   SDIX
  

Product name

 HPA019032   HPA057273   2126  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 4   13   5  

References

 2      

VALIDATION SUMMARY
 
 

Antibody HPA019032

  

Antibody HPA057273

  

Antibody CAB014886

  
Immunocytochemistry

Approved   Approved    
Immunohistochemistry

Approved     Approved  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA019032

  

Antibody HPA057273

  

Antibody CAB014886

  

Standard validation

 Approved   Approved    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear membrane.   Immunofluorescent staining of human cell line CACO-2 shows localization to nucleus.    

Antibody dilution

 1:49   1:137    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA019032

  

Antibody HPA057273

  

Antibody CAB014886

  

Standard validation

 Approved     Approved  

     

Figure description

 Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.     Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.  

Expression

 RNA: detected in 5 tissues
Protein: detected in 60 cell types		     RNA: detected in 5 tissues
Protein: detected in 66 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:10     1:250  

Literature conformity

 No avaliable gene/protein characterization data.     No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.     Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA019032

  

Antibody HPA057273

  

Antibody CAB014886

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 235.3   235.3   235.3  

Antibody dilution

 1:250   1:460   1:500  

PROTEIN ARRAY
 
 

Antibody HPA019032

  

Antibody HPA057273

  

Antibody CAB014886

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:5500    

ANTIGEN INFORMATION
 
 

Antibody HPA019032

  

Antibody HPA057273

  

Antibody CAB014886

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Genetic immunization  

Length (aa)

 144   124    

Antigen sequence

 
VPPNPIATFNAPSKWPEPQSTVSYGLAVQGAIQILPLGSGHTPQSSSNSE
KNSLPPVMAISNVENEKQVHISFLPANTQGFPLAPERGLFHASLGIAQLS
QAGPSKSDRGSSQVSVTSTVHVVNTTVVTMPVPMVSTSSSSYTT
  
MNLDRTEVLFQNPESLTCNGFTMALRSTSLSRRLSQPPLVVAKSKKVPLS
KGLEKQHDCDYKILPALGVKHSENDSVPMQDTQVLPDIETLIGVQNPSLL
KGKSQETTQFWSQRVEDSKINIPT
  

											 
																				

																		

										
											
Matching transcripts

												
																					TET1-001 - ENSP00000362748 [100%] 

											 
																						TET1-001 - ENSP00000362748 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
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RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA019032

												
										 
																				
											
											
											
Antibody HPA057273

												
										 
																				
											
											
											
Antibody CAB014886

												
										 
																		

																	

										
											
1.

												
																						Promoter demethylation of nuclear factor-erythroid 2-related factor 2 gene in drug-resistant colon cancer cells

	Zhao XQ et al

	Oncol Lett 2015;10(3):1287-1292

			Application: WB				
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						5-Hydroxymethylcytosine discriminates between parathyroid adenoma and carcinoma

	Barazeghi E et al

	Clin Epigenetics 2016;8:31

			Application: IHC				
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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