We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line Hep G2 shows localization to vesicles.
Immunofluorescent staining of human cell line Hep G2 shows localization to cell junctions & vesicles.
Antibody dilution
1:8
1:30
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA011954
Antibody HPA012852
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human liver shows strong positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong positivity in hepatocytes.
Expression
RNA: detected in 22 tissues Protein: detected in 12 cell types
RNA: detected in 22 tissues Protein: detected in 10 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:125
1:500
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA011954
Antibody HPA012852
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400631)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400631)
Target mass (kDa)
33.2, 13
33.2, 29.1, 18.4
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA011954
Antibody HPA012852
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
