Fields »
ABOUT
HELP
BLOG
PNMT

ANTIBODY INFORMATION
 
 

Antibody HPA043412

  

Antibody HPA051005

  

Antibody CAB017029

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   SDIX
  

Product name

 HPA043412   HPA051005   2169  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 12   12   4  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA043412

  

Antibody HPA051005

  

Antibody CAB017029

  
Immunocytochemistry

  Approved    
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA043412

  

Antibody HPA051005

  

Antibody CAB017029

  

Standard validation

   Approved    

     

Figure description

   Immunofluorescent staining of human cell line SiHa shows localization to vesicles.    

Antibody dilution

   1:5    

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA043412

  

Antibody HPA051005

  

Antibody CAB017029

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in medullary cells.   Immunohistochemical staining of human adrenal gland shows cytoplasmic positivity in medullary cells.   Immunohistochemical staining of human adrenal gland shows cytoplasmic positivity in medullary cells.  

Expression

 RNA: detected in 15 tissues
Protein: detected in 1 cell types		   RNA: detected in 15 tissues
Protein: detected in 3 cell types		   RNA: detected in 15 tissues
Protein: detected in 13 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:125   1:1500  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA043412

  

Antibody HPA051005

  

Antibody CAB017029

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 30.9, 20.6   30.9, 20.6   30.9, 20.6, 14.9  

Antibody dilution

 1:250   1:80   1:500  

PROTEIN ARRAY
 
 

Antibody HPA043412

  

Antibody HPA051005

  

Antibody CAB017029

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA043412

  

Antibody HPA051005

  

Antibody CAB017029

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Genetic immunization  

Length (aa)

 69   72    

Antigen sequence

 
ECWQDKERQLRARVKRVLPIDVHQPQPLGAGSPAPLPADALVSAFCLEAV
SPDLASFQRALDHITTLLR
  
GHLLLIGALEESWYLAGEARLTVVPVSEEEVREALVRSGYKVRDLRTYIM
PAHLQTGVDDVKGVFFAWAQKV
  

											 
																				

																		

										
											
Matching transcripts

												
																					PNMT-001 - ENSP00000269582 [100%] 
PNMT-002 - ENSP00000377791 [100%] 

											 
																						PNMT-001 - ENSP00000269582 [100%] 
PNMT-002 - ENSP00000377791 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PNMT-001
PNMT-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.