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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Antibody dilution
1:47
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA018015
Antibody HPA029226
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human appendix shows cytoplasmic positivity in leukocytes.
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in immune cells.
Expression
RNA: detected in 37 tissues Protein: detected in 48 cell types
RNA: detected in 37 tissues Protein: detected in 52 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:400
1:25
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA018015
Antibody HPA029226
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400212)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
63.5
63.5
Antibody dilution
1:125
1:250
PROTEIN ARRAY
Antibody HPA018015
Antibody HPA029226
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
