CARM1 - Antibodies - The Human Protein Atlas

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CARM1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA043561

Antibody HPA048073

Antibody CAB032961

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA043561

HPA048073

sc-33176

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA043561

Antibody HPA048073

Antibody CAB032961

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA043561

Antibody HPA048073

Antibody CAB032961

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.

Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & cytosol.

Antibody dilution

1:4

1:26

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA043561

Antibody HPA048073

Antibody CAB032961

Standard validation

Supported

Figure description

Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.

Expression

RNA: detected in 37 tissues
Protein: detected in 75 cell types

Retrieval

HIER pH6

Antibody dilution

1:75

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA043561

Antibody HPA048073

Antibody CAB032961

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

65.9, 63.5

65.9, 63.5, 18.2, 12.5

Antibody dilution

1:250

1:130

1:500

PROTEIN ARRAY

Antibody HPA043561

Antibody HPA048073

Antibody CAB032961

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:1050

ANTIGEN INFORMATION

Antibody HPA043561

Antibody HPA048073

Antibody CAB032961

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

104

Antigen sequence

DVCVFKCSVSRETECSRVGKQSFIITLGCNSVLIQFATPNDFCSFYNILK
TCRGHTLERSVFSERTEESSAVQYFQFYGYLSQQQ

HLAPFTDEQLYMEQFTKANFWYQPSFHGVDLSALRGAAVDEYFRQPVVDT
FDIRILMAKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFD
VAFI

Matching transcripts

CARM1-002 - ENSP00000325690 [100%]
CARM1-003 - ENSP00000340934 [100%]

Other gene match

ANTIGEN VIEW

CARM1-002	CARM1-003

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.