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RIT1

ANTIBODY INFORMATION
 
 

Antibody HPA053249

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA053249  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 11  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA053249

  
Immunohistochemistry

Approved  
Western blot

Supported  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA053249

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human kidney shows distinct membranous positivity in cells in tubules.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 55 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:75  

Literature conformity

 Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA053249

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 27.1, 25.1, 21.6  

Antibody dilution

 1:100  

PROTEIN ARRAY
 
 

Antibody HPA053249

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2700  

ANTIGEN INFORMATION
 
 

Antibody HPA053249

  

Antigen

 Recombinant protein fragment  

Length (aa)

 54  

Antigen sequence

 
AYRYYIDDVFHALVREIRRKEKEAVLAMEKKSKPKNSVWKRLKSPFRKKK
DSVT
 

Matching transcripts

 RIT1-001 - ENSP00000357306 [100%]
RIT1-002 - ENSP00000357305 [100%]
RIT1-005 - ENSP00000441950 [100%]
 

Other gene match

  
ANTIGEN VIEW
RIT1-001
RIT1-002
RIT1-005
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.