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Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.
Antibody dilution
1:23
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA047894
Antibody HPA048334
Antibody HPA060705
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in subset of glandular cells.
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in subset of glandular cells.
Expression
RNA: detected in 9 tissues Protein: detected in 6 cell types
RNA: detected in 9 tissues Protein: detected in 4 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:50
1:75
Literature conformity
Not consistent with gene/protein characterization data.
Not consistent with gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA047894
Antibody HPA048334
Antibody HPA060705
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
19.4, 19.3
19.4, 19.3
19.4, 19.3
Antibody dilution
1:270
1:170
1:80
PROTEIN ARRAY
Antibody HPA047894
Antibody HPA048334
Antibody HPA060705
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
