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HSPD1

ANTIBODY INFORMATION
 
 

Antibody HPA001523

  

Antibody HPA050025

  

Antibody CAB002775

  

Antibody CAB072816

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Chemicon
   NCI-CPTC
  

Product name

 HPA001523   HPA050025   MAB3514   CPTC-HSPD1-1  

Host species

 Rabbit   Rabbit   Mouse   Rabbit  

Clonality

 pAb   pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known   Protein A/G  

Other gene match

        

Released in version

 2   12   1   14  

References

 2        

VALIDATION SUMMARY
 
 

Antibody HPA001523

  

Antibody HPA050025

  

Antibody CAB002775

  

Antibody CAB072816

  
Immunocytochemistry


Validated
  

Validated
   Supported    
Immunohistochemistry

Supported    

Validated
   Supported  
Western blot

Supported   Supported   Supported   Uncertain  
Protein array

Supported   Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001523

  

Antibody HPA050025

  

Antibody CAB002775

  

Antibody CAB072816

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA050025.		  
Validated

Antibody staining overlaps with antibody HPA001523.		      
 

Standard validation

 Supported   Supported   Supported    

       

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.   Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.   Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.    

Antibody dilution

 1:32   1:49   1:50    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001523

  

Antibody HPA050025

  

Antibody CAB002775

  

Antibody CAB072816

  

Formal validation: Orthogonal

    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

   
 

 

    

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.    
 

Standard validation

 Supported     Supported   Supported  

       

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of renal tubules.     Immunohistochemical staining of human rectum shows strong cytoplasmic positivity, with a granular pattern, in glandular cells.   Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 68 cell types		     RNA: detected in 37 tissues
Protein: detected in 50 cell types		   RNA: detected in 37 tissues
Protein: detected in 76 cell types		  

Retrieval

 HIER pH6     HIER pH6   HIER pH6  

Antibody dilution

 1:400     1:200   1:35000  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.     Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001523

  

Antibody HPA050025

  

Antibody CAB002775

  

Antibody CAB072816

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

       

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 61.1, 25.2, 23.8, 19.2   61.1   61.1, 25.2, 23.8, 19.2, 10.3, 9, 6.4   61.1, 25.2, 23.8, 19.2, 10.3, 9, 6.4  

Antibody dilution

 1:250   1:250   1:1400   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001523

  

Antibody HPA050025

  

Antibody CAB002775

  

Antibody CAB072816

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:2000   1:30000      

ANTIGEN INFORMATION
 
 

Antibody HPA001523

  

Antibody HPA050025

  

Antibody CAB002775

  

Antibody CAB072816

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known   Recombinant protein  

Length (aa)

 140   135      

Antigen sequence

 
RVLAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIE
QSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTT
ATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAEL
  
KGVITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVL
LSEKKISSIQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGL
QVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGL
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					HSPD1-001 - ENSP00000373620 [100%] 
HSPD1-006 - ENSP00000412717 [100%] 
HSPD1-013 - ENSP00000393670 [100%] 
HSPD1-201 - ENSP00000340019 [100%] 
HSPD1-009 - ENSP00000414446 [84%] 

											 
																						HSPD1-001 - ENSP00000373620 [100%] 
HSPD1-201 - ENSP00000340019 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
HSPD1-001
HSPD1-006
HSPD1-009
HSPD1-013
HSPD1-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA001523

												
										 
																				
											
											
											
Antibody HPA050025

												
										 
																				
											
											
											
Antibody CAB002775

												
										 
																				
											
											
											
Antibody CAB072816

												
										 
																		

																	

										
											
1.

												
																						A global view of protein expression in human cells, tissues, and organs

	Pontén F et al

	Mol Syst Biol 2009;5:337

			
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy

	Stadler C et al

	J Proteomics 2012;75(7):2236-51

			
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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