CCNA2 - Antibodies - The Human Protein Atlas

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CCNA2

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA020626

Antibody CAB000114

Provider

Atlas Antibodies
Sigma-Aldrich

Novocastra

Product name

HPA020626

NCL-CYCLIN A

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Supernatant

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA020626

Antibody CAB000114

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA020626

Antibody CAB000114

Formal validation: Tagged protein

Validated

Antibody staining overlaps with GFP tagged protein

Figure description

Immunofluorescent staining of transgenic HeLa cells show antibody staining in cytoplasm & nucleoplasm and GFP expression in cytoplasm & nucleoplasm.

Antibody dilution

1:161

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.

Antibody dilution

1:161

1:50

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA020626

Antibody CAB000114

Standard validation

Approved

Figure description

Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in lymphoid cells outside reaction centra.

Immunohistochemical staining of human tonsil shows nuclear positivity in fractions of germinal center and non-germinal center cells.

Expression

RNA: detected in 30 tissues
Protein: detected in 25 cell types

RNA: detected in 30 tissues
Protein: detected in 35 cell types

Retrieval

HIER pH6

Antibody dilution

1:150

1:125

Literature conformity

Not consistent with gene/protein characterization data.

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA020626

Antibody CAB000114

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

48.6

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA020626

Antibody CAB000114

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA020626

Antibody CAB000114

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

PVNDEHVTVPPWKANSKQPAFTIHVDEAEKEAQKKPAESQKIEREDALAF
NSAISLPGPRKPLVPL

Matching transcripts

CCNA2-001 - ENSP00000274026 [100%]
CCNA2-201 - ENSP00000481380 [100%]

Other gene match

ANTIGEN VIEW

CCNA2-001	CCNA2-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.