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Immunofluorescent staining of human cell line A-431 shows localization to cytosol & the Golgi apparatus.
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Antibody dilution
1:20
1:200
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA029747
Antibody HPA049659
Standard validation
Approved
Figure description
Immunohistochemical staining of human placenta shows strong cytoplasmic and nuclear positivity in trophoblastic cells and decidual cells.
Expression
RNA: detected in 37 tissues Protein: detected in 75 cell types
Retrieval
HIER pH6
Antibody dilution
1:50
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA029747
Antibody HPA049659
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
48.9
48.9, 11.3
Antibody dilution
1:250
1:1020
PROTEIN ARRAY
Antibody HPA029747
Antibody HPA049659
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
