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TAF1

ANTIBODY INFORMATION
 
 

Antibody HPA001075

  

Antibody CAB016283

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA001075   sc-735  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 1   4  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA001075

  

Antibody CAB016283

  
Immunocytochemistry

Supported   Supported  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001075

  

Antibody CAB016283

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.  

Antibody dilution

 1:20   1:10  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001075

  

Antibody CAB016283

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human rectum shows nuclear positivity in glandular cells.   Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 42 cell types		   RNA: detected in 37 tissues
Protein: detected in 77 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:40  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001075

  

Antibody CAB016283

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 214.9, 214.7, 212.7   214.9, 214.7, 212.7, 32.4, 17.8  

Antibody dilution

 1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001075

  

Antibody CAB016283

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA001075

  

Antibody CAB016283

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 123    

Antigen sequence

 
SDSDVGSGGIRPKQPRMLQENTRMDMENEESMMSYEGDGGEASHGLEDSN
ISYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEEQRSGPSVLSQV
HLSEDEEDSEDFHSIAGDSDLDS
  

											 
																				

																		

										
											
Matching transcripts

												
																					TAF1-008 - ENSP00000276072 [100%] 
TAF1-009 - ENSP00000362895 [100%] 
TAF1-201 - ENSP00000406549 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
TAF1-008
TAF1-009
TAF1-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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