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MTA2

ANTIBODY INFORMATION
 
 

Antibody HPA006214

  

Antibody CAB005315

  

Antibody CAB017522

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
   Santa Cruz Biotechnology
  

Product name

 HPA006214   sc-9447   sc-55566  

Host species

 Rabbit   Goat   Mouse  

Clonality

 pAb   msAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity   Protein A/G  

Other gene match

      

Released in version

 3   3   4  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA006214

  

Antibody CAB005315

  

Antibody CAB017522

  
Immunocytochemistry


Validated
     Supported  
Immunohistochemistry

Supported   Supported   Supported  
Western blot


Validated
   Uncertain   Supported  
Protein array

Approved      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA006214

  

Antibody CAB005315

  

Antibody CAB017522

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		      

 

Relative fluorescence intensity (RFI)

      

Figure description

 Nuclear region of segmented cells in 10x-images      

Antibody dilution

 1:75      
 

Standard validation

 Supported     Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.     Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.  

Antibody dilution

 1:74     1:13  

Literature conformity

 The subcellular location is supported by literature.     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA006214

  

Antibody CAB005315

  

Antibody CAB017522

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human tonsil shows strong nuclear positivity.   Immunohistochemical staining of human tonsil shows strong nuclear positivity.   Immunohistochemical staining of human tonsil shows strong nuclear positivity.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 78 cell types		   RNA: detected in 37 tissues
Protein: detected in 79 cell types		   RNA: detected in 37 tissues
Protein: detected in 71 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:50   1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA006214

  

Antibody CAB005315

  

Antibody CAB017522

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		      

     

Figure description

 Lane 1: Marker [kDa] 250, 150, 100, 75, 50, 37, 25, 20, 15, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		      

Target mass (kDa)

 75, 55      

Loading control

 Total protein image      

Antibody dilution

 1:170      
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 75, 55   75, 55   75, 55  

Antibody dilution

 1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA006214

  

Antibody CAB005315

  

Antibody CAB017522

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA006214

  

Antibody CAB005315

  

Antibody CAB017522

  

Antigen

 Recombinant protein fragment   Synthetic peptide   Not known  

Length (aa)

 148      

Antigen sequence

 
ECSIRLPKAAKTPLKIHPLVRLPLATIVKDLVAQAPLKPKTPRGTKTPIN
RNQLSQNRGLGGIMVKRAYETMAGAGVPFSANGRPLASGIRSSSQPAAKR
QKLNPADAPNPVVFVATKDTRALRKALTHLEMRRAARRPNLPLKVKPT
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					MTA2-001 - ENSP00000278823 [100%] 
MTA2-002 - ENSP00000431346 [100%] 
MTA2-003 - ENSP00000431797 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
MTA2-001
MTA2-002
MTA2-003
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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