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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & microtubules.
Antibody dilution
1:182
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA038562
Standard validation
Approved
Figure description
Immunohistochemical staining of human stomach shows string cytoplasmic and membrane positivity in glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 63 cell types
Retrieval
HIER pH6
Antibody dilution
1:450
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA038562
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416424)
Target mass (kDa)
22.5
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA038562
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
