We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line HaCaT shows localization to nucleus, nuclear bodies & cytosol.
Antibody dilution
1:200
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA049022
Antibody HPA057259
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human colon shows nuclear positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 23 cell types
RNA: detected in 37 tissues Protein: detected in 58 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:3500
1:450
Literature conformity
Not consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA049022
Antibody HPA057259
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
175
175
Antibody dilution
1:1050
1:190
PROTEIN ARRAY
Antibody HPA049022
Antibody HPA057259
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
