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SLC16A1

ANTIBODY INFORMATION
 
 

Antibody HPA003324

  

Antibody HPA071055

  

Antibody CAB017489

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA003324   HPA071055   sc-50324  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 5   16   4  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA003324

  

Antibody HPA071055

  

Antibody CAB017489

  
Immunocytochemistry


Validated
   Supported   Supported  
Immunohistochemistry


Validated
      
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003324

  

Antibody HPA071055

  

Antibody CAB017489

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by one siRNA.		      

 

Relative fluorescence intensity (RFI)

      

Figure description

 Cytoplasmic region of segmented cells in 10x-images      

Antibody dilution

 1:46      
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.   Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane, cell junctions & vesicles.   Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.  

Antibody dilution

 1:47   1:56   1:87  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003324

  

Antibody HPA071055

  

Antibody CAB017489

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		      

 

 

      

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.      
 

Standard validation

 Supported      

     

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.      

Expression

 RNA: detected in 35 tissues
Protein: detected in 42 cell types		      

Retrieval

 HIER pH6      

Antibody dilution

 1:350      

Literature conformity

 Partly consistent with extensive gene/protein characterization data.      

RNA consistency

 Mainly consistent with RNA expression data.      

WESTERN BLOT
 
 

Antibody HPA003324

  

Antibody HPA071055

  

Antibody CAB017489

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 53.9, 49.2, 31.7   53.9   53.9, 49.2, 31.7, 22.5  

Antibody dilution

 1:250   1:350   1:500  

PROTEIN ARRAY
 
 

Antibody HPA003324

  

Antibody HPA071055

  

Antibody CAB017489

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:2250    

ANTIGEN INFORMATION
 
 

Antibody HPA003324

  

Antibody HPA071055

  

Antibody CAB017489

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 62   51    

Antigen sequence

 
PTKAGKDKSKASLEKAGKSGVKKDLHDANTDLIGRHPKQEKRSVFQTINQ
FLDLTLFTHRGF
  
KEQKANEQKKESKEEETSIDVAGKPNEVTKAAESPDQKDTDGGPKEEESP
V
  

											 
																				

																		

										
											
Matching transcripts

												
																					SLC16A1-001 - ENSP00000358640 [100%] 
SLC16A1-002 - ENSP00000416167 [100%] 
SLC16A1-005 - ENSP00000399104 [100%] 
SLC16A1-201 - ENSP00000441065 [100%] 

											 
																						SLC16A1-001 - ENSP00000358640 [100%] 
SLC16A1-201 - ENSP00000441065 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
SLC16A1-001
SLC16A1-002
SLC16A1-005
SLC16A1-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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