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ANTIBODY INFORMATION
 
 

Antibody HPA018859

  

Antibody HPA019938

  

Antibody HPA025230

  

Antibody CAB002679

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Lab Vision/NeoMarkers
  

Product name

 HPA018859   HPA019938   HPA025230   RB-1580  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

        

Released in version

 5   5   5   1  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA018859

  

Antibody HPA019938

  

Antibody HPA025230

  

Antibody CAB002679

  
Immunocytochemistry

  Supported      
Immunohistochemistry

Supported   Supported   Supported   Supported  
Western blot

Supported   Supported   Supported   Supported  
Protein array

Supported   Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA018859

  

Antibody HPA019938

  

Antibody HPA025230

  

Antibody CAB002679

  

Standard validation

   Supported      

       

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.      

Antibody dilution

   1:400      

Literature conformity

   The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA018859

  

Antibody HPA019938

  

Antibody HPA025230

  

Antibody CAB002679

  

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human urinary bladder shows nuclear positivity in urothelial cells.   Immunohistochemical staining of human urinary bladder shows nuclear and cytoplasmic positivity in urothelial cells.   Immunohistochemical staining of human urinary bladder shows nuclear and cytoplasmic positivity in urothelial cells.   Immunohistochemical staining of human urinary bladder shows nuclear positivity in urothelial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 67 cell types		   RNA: detected in 37 tissues
Protein: detected in 61 cell types		   RNA: detected in 37 tissues
Protein: detected in 75 cell types		   RNA: detected in 37 tissues
Protein: detected in 46 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:1200   1:200   1:150  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA018859

  

Antibody HPA019938

  

Antibody HPA025230

  

Antibody CAB002679

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

       

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 36.5, 29.4   36.5, 29.4   36.5, 29.4   36.5, 29.4  

Antibody dilution

 1:250   1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA018859

  

Antibody HPA019938

  

Antibody HPA025230

  

Antibody CAB002679

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA018859

  

Antibody HPA019938

  

Antibody HPA025230

  

Antibody CAB002679

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 80   85   73    

Antigen sequence

 
MEVTGDAGVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRSKACDIL
AIDKSLTPVTLVLAEDGTIVDDDDYFLCLP
  
LRQSCAIVQRLQHTLQQVLDQREEVRQSKQLLQLYLQALEKEGSLLSKQE
ESKAAFGEEVDAVDTGISRETSSDVALASHILTAL
  
TKFVALASNEKWAYNNSDGGTAWISQESFDVDETDSGAGLKWKNVARQLK
EDLSSIILLSEEDLQMLVDAPCS
  

											 
																				

																		

										
											
Matching transcripts

												
																					DFFA-001 - ENSP00000366237 [100%] 
DFFA-002 - ENSP00000366235 [100%] 

											 
																						DFFA-001 - ENSP00000366237 [99%] 
DFFA-002 - ENSP00000366235 [99%] 

											 
																						DFFA-001 - ENSP00000366237 [100%] 
DFFA-002 - ENSP00000366235 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
DFFA-001
DFFA-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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