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DFFA

GENERAL INFORMATION

Gene name

 DFFA

Gene description

 DNA fragmentation factor, 45kDa, alpha polypeptide

Protein class

 Plasma proteins
Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 2
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HUMAN PROTEIN ATLAS INFORMATION

RNA tissue category
HPA:Expressed in all
GTEx:Expressed in all
FANTOM5:Expressed in all

Protein evidence

 Evidence at protein level

Protein expression

 Ubiquitous nuclear expression often combined with cytoplasmic expression.

DATA RELIABILITY

Data reliability
description

 Antibody staining mainly consistent with RNA expression data.

Reliability score

 Supported based on 4 antibodies.
HPA018859 , HPA019938 , HPA025230 , CAB002679
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RNA AND PROTEIN EXPRESSION SUMMARY
RNA expression (TPM) Protein expression (score)
Brain
Caudate
N/A
Endocrine tissues
Bone marrow & immune system
Muscle tissues
Lung
Bronchus
N/A
Liver & gallbladder
Pancreas
Gastrointestinal tract
Kidney & urinary bladder
Male tissues
Female tissues
Vagina
N/A
Adipose & soft tissue
Skin
Cerebral cortex

Lymph node

Liver

Gallbladder

Colon

Kidney

Testis

PROTEIN EXPRESSION OVERVIEW
Organ
Expression
Alphabetical

RNA EXPRESSION OVERVIEW
HPA dataset
Organ
Expression
Alphabetical
RNA tissue category: Expressed in all
GTEx dataset
Organ
Expression
Alphabetical
RNA tissue category: Expressed in all
FANTOM5 dataset
Organ
Expression
Alphabetical
RNA tissue category: Expressed in all

GENE INFORMATION

Gene name

 DFFA (HGNC Symbol)

Synonyms

 DFF-45, DFF1, DFF45, ICAD

Description

 DNA fragmentation factor, 45kDa, alpha polypeptide (HGNC Symbol)

Entrez gene summary

 Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Chromosome

 1

Cytoband

 p36.22

Chromosome location (bp)

 10456522 - 10472526

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000160049 (version 83.38)

Entrez gene

 1676

UniProt

 O00273 (UniProt - Evidence at protein level)

neXtProt

 NX_O00273

Antibodypedia

 DFFA antibodies

PROTEIN BROWSER
DFFA-001
DFFA-002

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
DFFA-001
ENSP00000366237
ENST00000377038
O00273 [Direct mapping]
DNA fragmentation factor subunit alpha
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Predicted intracellular proteins
Plasma proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000790 [nuclear chromatin]
GO:0005515 [protein binding]
GO:0005622 [intracellular]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0006309 [apoptotic DNA fragmentation]
GO:0006915 [apoptotic process]
GO:0006921 [cellular component disassembly involved in execution phase of apoptosis]
GO:0012501 [programmed cell death]
GO:0043065 [positive regulation of apoptotic process]
GO:0070242 [thymocyte apoptotic process]
GO:1900118 [negative regulation of execution phase of apoptosis]
GO:1902511 [negative regulation of apoptotic DNA fragmentation]
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 331 aa
36.5 kDa 		No 0
DFFA-002
ENSP00000366235
ENST00000377036
O00273 [Direct mapping]
DNA fragmentation factor subunit alpha
A0A024R4H5 [Target identity:100%; Query identity:100%]
DNAation factor, 45kDa, alpha polypeptide, isoform CRA_b
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Predicted intracellular proteins
Plasma proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000790 [nuclear chromatin]
GO:0005515 [protein binding]
GO:0005622 [intracellular]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0006309 [apoptotic DNA fragmentation]
GO:0006915 [apoptotic process]
GO:0006921 [cellular component disassembly involved in execution phase of apoptosis]
GO:0012501 [programmed cell death]
GO:1900118 [negative regulation of execution phase of apoptosis]
GO:1902511 [negative regulation of apoptotic DNA fragmentation]
Show all
 268 aa
29.4 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.