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Antibody staining overlaps with antibody HPA025820.
Validated
Antibody staining overlaps with antibody HPA006811.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Antibody dilution
1:29
1:50
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA006811
Antibody HPA025820
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA025820 across 72 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA006811 across 72 cell types.
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 34 cell types
RNA: detected in 37 tissues Protein: detected in 32 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:35
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA006811
Antibody HPA025820
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
61.6, 58
61.6, 58
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA006811
Antibody HPA025820
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
