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Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
Antibody dilution
1:133
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA016591
Standard validation
Approved
Figure description
Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 61 cell types
Retrieval
HIER pH6
Antibody dilution
1:10
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA016591
Standard validation
Uncertain
Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409544)
Target mass (kDa)
36.5, 36.2, 34, 30.7, 16.5
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA016591
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
