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RBM15

ANTIBODY INFORMATION
 
 

Antibody HPA019824

  

Antibody HPA049642

  

Antibody CAB015201

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   SDIX
  

Product name

 HPA019824   HPA049642   2139  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 5   12   4  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA019824

  

Antibody HPA049642

  

Antibody CAB015201

  
Immunocytochemistry


Validated
  

Validated
   Supported  
Immunohistochemistry

Supported     Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA019824

  

Antibody HPA049642

  

Antibody CAB015201

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA049642.		  
Validated

Antibody staining overlaps with antibody HPA019824.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.  

Antibody dilution

 1:50   1:67   1:50  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA019824

  

Antibody HPA049642

  

Antibody CAB015201

  

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.     Immunohistochemical staining of human gall bladder shows cytoplasmic and nuclear positivity in glandular cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 56 cell types		     RNA: detected in 36 tissues
Protein: detected in 70 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:10     1:200  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA019824

  

Antibody HPA049642

  

Antibody CAB015201

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 107.2, 106.4, 104.8, 102.1   107.2, 106.4, 104.8, 102.1   107.2, 106.4, 104.8, 102.1  

Antibody dilution

 1:250   1:340   1:500  

PROTEIN ARRAY
 
 

Antibody HPA019824

  

Antibody HPA049642

  

Antibody CAB015201

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:2700    

ANTIGEN INFORMATION
 
 

Antibody HPA019824

  

Antibody HPA049642

  

Antibody CAB015201

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Genetic immunization  

Length (aa)

 115   106    

Antigen sequence

 
DTYPPSASVVGASVGGHRHPPGGGGGQRSLSPGGAALGYRDYRLQQLALG
RLPPPPPPPLPRDLERERDYPFYERVRPAYSLEPRVGAGAGAAPFREVDE
ISPEDDQRANRTLFL
  
RGARDRTPPLLYRDRDRDLYPDSDWVPPPPPVRERSTRTAATSVPAYEPL
DSLDRRRDGWSLDRDRGDRDLPSSRDQPRKRRLPEESGGRHLDRSPESDR
PRKRHC
  

											 
																				

																		

										
											
Matching transcripts

												
																					RBM15-001 - ENSP00000358799 [100%] 
RBM15-002 - ENSP00000473552 [100%] 
RBM15-003 - ENSP00000473638 [100%] 
RBM15-004 - ENSP00000480399 [100%] 
RBM15-201 - ENSP00000483133 [100%] 

											 
																						RBM15-001 - ENSP00000358799 [100%] 
RBM15-002 - ENSP00000473552 [100%] 
RBM15-003 - ENSP00000473638 [100%] 
RBM15-004 - ENSP00000480399 [100%] 
RBM15-201 - ENSP00000483133 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
RBM15-001
RBM15-002
RBM15-003
RBM15-004
RBM15-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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