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Immunofluorescent staining of human cell line PC-3 shows localization to cytosol.
Antibody dilution
1:52
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA065983
Antibody HPA071879
Standard validation
Approved
Figure description
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules.
Expression
RNA: detected in 37 tissues Protein: detected in 62 cell types
Retrieval
HIER pH6
Antibody dilution
1:800
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA065983
Antibody HPA071879
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
148.5, 86.8
148.5, 86.8
Antibody dilution
1:300
1:170
PROTEIN ARRAY
Antibody HPA065983
Antibody HPA071879
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
