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Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Antibody dilution
1:7
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA037679
Antibody HPA037680
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Expression
RNA: detected in 37 tissues Protein: detected in 68 cell types
RNA: detected in 37 tissues Protein: detected in 72 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:25
1:25
Literature conformity
Consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA037679
Antibody HPA037680
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY417924)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY417924)
Target mass (kDa)
20.9
20.9
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA037679
Antibody HPA037680
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
