SUN1 - Antibodies - The Human Protein Atlas

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SUN1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA008346

Antibody HPA008461

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA008346

HPA008461

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA008346

Antibody HPA008461

Immunocytochemistry

Supported

Immunohistochemistry

Western blot

Supported

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA008346

Antibody HPA008461

Formal validation: Genetic

Validated

Significant downregulation > 25% by one siRNA and > 10% by the other.

Relative fluorescence intensity (RFI)

Figure description

Nuclear region of segmented cells in 10x-images

Antibody dilution

1:59

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nuclear membrane.

Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & vesicles.

Antibody dilution

1:61

1:91

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA008346

Antibody HPA008461

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA008461 across 67 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA008346 across 67 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human placenta shows strong nuclear membrane positivity in trophoblastic cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 71 cell types

RNA: detected in 37 tissues
Protein: detected in 70 cell types

Retrieval

HIER pH6

Antibody dilution

1:250

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA008346

Antibody HPA008461

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

91.1, 87.1, 79.6, 77.9, 76.4, 70.9

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA008346

Antibody HPA008461

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA008346

Antibody HPA008461

Antigen

Recombinant protein fragment

Length (aa)

132

136

Antigen sequence

QLLPTVEHLQLELDQLKSELSSWRHVKTGCETVDAVQERVDVQVREMVKL
LFSEDQQGGSLEQLLQRFSSQFVSKGDLQTMLRDLQLQILRNVTHHVSVT
KQLPTSEAVVSAVSEAGASGITEAQARAIVNS

QGNFFSFLPVLNWASMHRTQRVDDPQDVFKPTTSRLKQPLQGDSEAFPWH
WMSGVEQQVASLSGQCHHHGENLRELTTLLQKLQARVDQMEGGAAGPSAS
VRDAVGQPPRETDFMAFHQEHEVRMSHLEDILGKLR

Matching transcripts

SUN1-001 - ENSP00000384116 [100%]
SUN1-002 - ENSP00000374225 [100%]
SUN1-005 - ENSP00000384015 [100%]
SUN1-008 - ENSP00000409909 [100%]
SUN1-012 - ENSP00000406653 [100%]
SUN1-201 - ENSP00000392309 [100%]
SUN1-202 - ENSP00000413439 [100%]

Other gene match

ANTIGEN VIEW

SUN1-001	SUN1-002	SUN1-005	SUN1-008	SUN1-012	SUN1-201	SUN1-202

RELEVANT PUBLICATIONS

Antibody HPA008346

Antibody HPA008461

Membrane insertion of the pleckstrin homology domain variable loop 1 is critical for dynamin-catalyzed vesicle scission
Ramachandran R et al
Mol Biol Cell 2009;20(22):4630-9

Nuclear envelope-associated endosomes deliver surface proteins to the nucleus
Chaumet A et al
Nat Commun 2015;6:8218
Application: ICC-IF

Induction of a massive endoplasmic reticulum and perinuclear space expansion by expression of lamin B receptor mutants and the related sterol reductases TM7SF2 and DHCR7
Zwerger M et al
Mol Biol Cell 2010;21(2):354-68

Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy
Stadler C et al
J Proteomics 2012;75(7):2236-51

Human telomeres are tethered to the nuclear envelope during postmitotic nuclear assembly
Crabbe L et al
Cell Rep 2012;2(6):1521-9

A mammalian KASH domain protein coupling meiotic chromosomes to the cytoskeleton
Horn HF et al
J Cell Biol 2013;202(7):1023-39

Muscular dystrophy-associated SUN1 and SUN2 variants disrupt nuclear-cytoskeletal connections and myonuclear organization
Meinke P et al
PLoS Genet 2014;10(9):e1004605
Application: ICC-IF, IP, WB

Samp1 is functionally associated with the LINC complex and A-type lamina networks
Gudise S et al
J Cell Sci 2011;124(Pt 12):2077-85
Application: ICC-IF

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.