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Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Expression
RNA: detected in 7 tissues Protein: detected in 3 cell types
RNA: detected in 7 tissues Protein: detected in 45 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:500
1:600
Literature conformity
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA040842
Antibody HPA040884
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412545)
Target mass (kDa)
39.9, 37.1, 36.2, 33.5
39.9, 37.1, 36.2, 33.5
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA040842
Antibody HPA040884
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
