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Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Antibody dilution
1:20
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA016429
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human fallopian tube shows cytoplasmic and nuclear positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 74 cell types
Retrieval
HIER pH6
Antibody dilution
1:15
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA016429
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
56, 51.2
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA016429
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:500
ANTIGEN INFORMATION
Antibody HPA016429
Antigen
Recombinant protein fragment
Length (aa)
151
Antigen sequence
INKLLKYLDLEEEADTTKAFDLRQNHSILKIEKVLKRMREIKNELLQAQN
PSELYLSSKTELQGLIGQLDEVSLEKNPCIREARRRAVIEVQTLITYIDL
KEALEKRKLFACEEHPSHKAVWNVLGNLSEIQGEVLSFDGNRTDKNYIRL
E
