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HSP90B1

ANTIBODY INFORMATION
 
 

Antibody HPA003901

  

Antibody HPA008424

  

Antibody CAB005224

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA003901   HPA008424   sc-1794  

Host species

 Rabbit   Rabbit   Goat  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 4   4   3  

References

 2      

VALIDATION SUMMARY
 
 

Antibody HPA003901

  

Antibody HPA008424

  

Antibody CAB005224

  
Immunocytochemistry


Validated
  

Validated
   Supported  
Immunohistochemistry

Supported   Supported   Supported  
Western blot


Validated
   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003901

  

Antibody HPA008424

  

Antibody CAB005224

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA008424.		  
Validated

Antibody staining overlaps with antibody HPA003901.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.   Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.   Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.  

Antibody dilution

 1:200   1:24   1:62  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003901

  

Antibody HPA008424

  

Antibody CAB005224

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 69 cell types		   RNA: detected in 37 tissues
Protein: detected in 74 cell types		   RNA: detected in 37 tissues
Protein: detected in 78 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:3000   1:450   1:250  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA003901

  

Antibody HPA008424

  

Antibody CAB005224

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		      

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		      

Target mass (kDa)

 92.5, 16.9      

Loading control

 Total protein image      

Antibody dilution

 1:442      
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 92.5, 16.9   92.5   92.5, 35.4, 16.9, 5.6  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA003901

  

Antibody HPA008424

  

Antibody CAB005224

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:10000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA003901

  

Antibody HPA008424

  

Antibody CAB005224

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 144   111    

Antigen sequence

 
SGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIRDMLRRIKED
EDDKTVLDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNIDPDAK
VEEEPEEEPEETAEDTTEDTEQDEDEEMDVGTDEEEETAKESTA
  
WSSKTETVEEPMEEEEAAKEEKEESDDEAAVEEEEEEKKPKTKKVEKTVW
DWELMNDIKPIWQRPSKEVEEDEYKAFYKSFSKESDDPMAYIHFTAEGEV
TFKSILFVPTS
  

											 
																				

																		

										
											
Matching transcripts

												
																					HSP90B1-001 - ENSP00000299767 [100%] 
HSP90B1-012 - ENSP00000450215 [98%] 

											 
																						HSP90B1-001 - ENSP00000299767 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
HSP90B1-001
HSP90B1-012
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA003901

												
										 
																				
											
											
											
Antibody HPA008424

												
										 
																				
											
											
											
Antibody CAB005224

												
										 
																		

																	

										
											
1.

												
																						Proteomic analyses reveal high expression of decorin and endoplasmin (HSP90B1) are associated with breast cancer metastasis and decreased survival

	Cawthorn TR et al

	PLoS One 2012;7(2):e30992

			
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Novel application for pseudopodia proteomics using excimer laser ablation and two-dimensional difference gel electrophoresis

	Ito A et al

	Lab Invest 2012;92(9):1374-85

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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