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ZNF598

ANTIBODY INFORMATION
 
 

Antibody HPA041760

  

Antibody HPA041896

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA041760   HPA041896  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 8   8  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA041760

  

Antibody HPA041896

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA041760

  

Antibody HPA041896

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.   Immunofluorescent staining of human cell line A-431 shows localization to cytosol.  

Antibody dilution

 1:192   1:92  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041760

  

Antibody HPA041896

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 74 cell types		   RNA: detected in 36 tissues
Protein: detected in 73 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:75  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA041760

  

Antibody HPA041896

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 98.8, 93.3   98.8, 93.3  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA041760

  

Antibody HPA041896

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA041760

  

Antibody HPA041896

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 114   81  

Antigen sequence

 
VVGGEDYEEVDRYSRQGRVARAGTRGAQQSRRGSWRYKREEEDREVAAAV
RASVAAQQQEEARRSEDQEEGGRPKKEEAAARGPEDPRGPRRSPRTQGEG
PGPKETSTNGPVSQ
  
TTTTTKAPRLLPAPRAYLVPENFRERNLQLIQSIRDFLQSDEARFSEFKS
HSGEFRQGLISAAQYYKSCRDLLGENFQKVF
 

Matching transcripts

 ZNF598-001 - ENSP00000455882 [100%]
ZNF598-004 - ENSP00000455308 [100%]
ZNF598-201 - ENSP00000411409 [100%]
  ZNF598-001 - ENSP00000455882 [100%]
ZNF598-004 - ENSP00000455308 [100%]
ZNF598-201 - ENSP00000411409 [100%]
 

Other gene match

    
ANTIGEN VIEW
ZNF598-001
ZNF598-004
ZNF598-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.