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Immunofluorescent staining of human cell line RT4 shows localization to nucleus & vesicles.
Antibody dilution
1:25
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA042713
Antibody HPA056752
Standard validation
Approved
Figure description
Immunohistochemical staining of human small intestine shows moderate luminal membranous positivity in glandular cells.
Expression
RNA: detected in 15 tissues Protein: detected in 27 cell types
Retrieval
HIER pH6
Antibody dilution
1:1000
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA042713
Antibody HPA056752
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
95.1
95.1
Antibody dilution
1:250
1:260
PROTEIN ARRAY
Antibody HPA042713
Antibody HPA056752
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
