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Immunohistochemical staining of human epididymis shows distinct luminal membranous positivity in glandular cells and extracellular positivity.
Immunohistochemical staining of human epididymis shows membranous positivity in glandular cells.
Expression
RNA: detected in 3 tissues Protein: detected in 30 cell types
RNA: detected in 3 tissues Protein: detected in 6 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:2000
1:200
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA044256
Antibody HPA050341
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY411747)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
26.1, 25.6, 17.9, 17.5
26.1, 25.6, 17.9, 8.9
Antibody dilution
1:125
1:180
PROTEIN ARRAY
Antibody HPA044256
Antibody HPA050341
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
