Fields »
ABOUT
HELP
BLOG
CHRNA5

ANTIBODY INFORMATION
 
 

Antibody HPA054381

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA054381  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 11  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA054381

  
Immunocytochemistry

Approved  
Immunohistochemistry

Approved  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA054381

  

Standard validation

 Approved  

 

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & focal adhesion sites.  

Antibody dilution

 1:43  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA054381

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human hippocampus shows strong cytoplasmic and membranous positivity in processes and blood vessels.  

Expression

 RNA: detected in 19 tissues
Protein: detected in 42 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:75  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA054381

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

 

Figure description

  

Target mass (kDa)

 53.1, 17.8  

Antibody dilution

 1:210  

PROTEIN ARRAY
 
 

Antibody HPA054381

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1700  

ANTIGEN INFORMATION
 
 

Antibody HPA054381

  

Antigen

 Recombinant protein fragment  

Length (aa)

 39  

Antigen sequence

 
AQRGLSEPSSIAKHEDSLLKDLFQDYERWVRPVEHLNDK
 

Matching transcripts

 CHRNA5-001 - ENSP00000299565 [100%]
CHRNA5-003 - ENSP00000453519 [100%]
 

Other gene match

  
ANTIGEN VIEW
CHRNA5-001
CHRNA5-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.