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Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, the Golgi apparatus & cell junctions.
Antibody dilution
1:75
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA015077
Antibody CAB037339
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic and nuclear positivity in neuronal cells, neuropil displayed moderate cytoplasmic staining.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells and neuropil.
Expression
RNA: detected in 10 tissues Protein: detected in 12 cell types
RNA: detected in 10 tissues Protein: detected in 60 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1200
1:250
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA015077
Antibody CAB037339
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
132.7, 123.1, 18.9, 18.4
132.7, 123.1, 98, 35.8, 22.7, 18.9, 18.4
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA015077
Antibody CAB037339
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
