Fields »
ABOUT
HELP
BLOG
UBB

ANTIBODY INFORMATION
 
 

Antibody HPA041344

  

Antibody HPA049132

  

Antibody CAB013048

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Zymed
  

Product name

 HPA041344   HPA049132   13-1600  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

 RPS27A - ENSG00000143947 [100%]
UBC - ENSG00000150991 [100%]
UBA52 - ENSG00000221983 [100%]
   RPS27A - ENSG00000143947 [97%]
UBC - ENSG00000150991 [100%]
UBA52 - ENSG00000221983 [97%]
    

Released in version

 10   10   4  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA041344

  

Antibody HPA049132

  

Antibody CAB013048

  
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Supported   Uncertain  
Protein array

Supported   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041344

  

Antibody HPA049132

  

Antibody CAB013048

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human oral mucosa shows strong nuclear positivity in squamous epithelial cells.   Immunohistochemical staining of human urinary bladder shows nuclear and cytoplasmic positivity in urothelial cells.   Immunohistochemical staining of human duodenum shows nuclear and cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 63 cell types		   RNA: detected in 37 tissues
Protein: detected in 76 cell types		   RNA: detected in 37 tissues
Protein: detected in 71 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:450   1:10000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA041344

  

Antibody HPA049132

  

Antibody CAB013048

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 77, 34.3, 25.8, 23.2, 19, 18.2, 18, 17.5, 17.2, 16.8, 15.3, 15, 14.7, 13.8, 12.2, 10.5, 7.1, 6.9, 4.9   77, 34.3, 25.8, 23.2, 19, 18.2, 18, 17.5, 17.2, 16.8, 15.3, 15, 14.7, 13.8, 12.2, 10.5, 7.1, 6.9, 4.9   25.8, 23.2, 17.2, 10.5, 4.9  

Antibody dilution

 1:250   1:270   1:500  

PROTEIN ARRAY
 
 

Antibody HPA041344

  

Antibody HPA049132

  

Antibody CAB013048

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:2200    

ANTIGEN INFORMATION
 
 

Antibody HPA041344

  

Antibody HPA049132

  

Antibody CAB013048

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 41   36    

Antigen sequence

 
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQ
  
GMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEG
  

											 
																				

																		

										
											
Matching transcripts

												
																					UBB-001 - ENSP00000304697 [100%] 
UBB-002 - ENSP00000379178 [100%] 
UBB-003 - ENSP00000379180 [100%] 
UBB-005 - ENSP00000437475 [100%] 
UBB-006 - ENSP00000464594 [100%] 
UBB-007 - ENSP00000464510 [100%] 
UBB-008 - ENSP00000463156 [100%] 
UBB-201 - ENSP00000478771 [100%] 

											 
																						UBB-001 - ENSP00000304697 [100%] 
UBB-002 - ENSP00000379178 [100%] 
UBB-003 - ENSP00000379180 [100%] 
UBB-005 - ENSP00000437475 [100%] 
UBB-008 - ENSP00000463156 [100%] 
UBB-201 - ENSP00000478771 [100%] 
UBB-006 - ENSP00000464594 [97%] 
UBB-007 - ENSP00000464510 [97%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					RPS27A - ENSG00000143947 [100%]
UBC - ENSG00000150991 [100%]
UBA52 - ENSG00000221983 [100%]

											 
																						RPS27A - ENSG00000143947 [97%]
UBC - ENSG00000150991 [100%]
UBA52 - ENSG00000221983 [97%]

											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
UBB-001
UBB-002
UBB-003
UBB-005
UBB-006
UBB-007
UBB-008
UBB-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.