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UBC

ANTIBODY INFORMATION
 
 

Antibody HPA041344

  

Antibody HPA049132

  

Antibody CAB000362

  

Antibody CAB005419

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   AbD Serotec
   Santa Cruz Biotechnology
  

Product name

 HPA041344   HPA049132   MCA1398GT   sc-9133  

Host species

 Rabbit   Rabbit   Mouse   Rabbit  

Clonality

 pAb   pAb   mAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known   Protein A/G  

Other gene match

 RPS27A - ENSG00000143947 [100%]
UBB - ENSG00000170315 [100%]
UBA52 - ENSG00000221983 [100%]
   RPS27A - ENSG00000143947 [97%]
UBB - ENSG00000170315 [100%]
UBA52 - ENSG00000221983 [97%]
      

Released in version

 10   10   1   2  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA041344

  

Antibody HPA049132

  

Antibody CAB000362

  

Antibody CAB005419

  
Immunocytochemistry

    Approved    
Immunohistochemistry

Supported   Supported   Supported   Supported  
Western blot

Supported   Supported   Uncertain   Uncertain  
Protein array

Supported   Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA041344

  

Antibody HPA049132

  

Antibody CAB000362

  

Antibody CAB005419

  

Standard validation

     Approved    

       

Figure description

     Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.    

Antibody dilution

     1:200    

Literature conformity

     The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041344

  

Antibody HPA049132

  

Antibody CAB000362

  

Antibody CAB005419

  

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human oral mucosa shows strong nuclear positivity in squamous epithelial cells.   Immunohistochemical staining of human urinary bladder shows nuclear and cytoplasmic positivity in urothelial cells.   Immunohistochemical staining of human oral mucosa shows strong nuclear positivity in squamous epithelial cells.   Immunohistochemical staining of human placenta shows distinct nuclear and cytoplasmic positivity in trophoblastic cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 63 cell types		   RNA: detected in 37 tissues
Protein: detected in 76 cell types		   RNA: detected in 37 tissues
Protein: detected in 58 cell types		   RNA: detected in 37 tissues
Protein: detected in 71 cell types		  

Retrieval

 HIER pH6   HIER pH6   None   HIER pH6  

Antibody dilution

 1:50   1:450   1:1000   1:100  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA041344

  

Antibody HPA049132

  

Antibody CAB000362

  

Antibody CAB005419

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 77, 34.3, 25.8, 23.2, 19, 18.2, 18, 17.5, 17.2, 16.8, 15.3, 15, 14.7, 13.8, 12.2, 10.5, 7.1, 6.9, 4.9   77, 34.3, 25.8, 23.2, 19, 18.2, 18, 17.5, 17.2, 16.8, 15.3, 15, 14.7, 13.8, 12.2, 10.5, 7.1, 6.9, 4.9   77, 34.3, 25.8, 19, 18.2, 17.5, 16.8, 15.3, 15, 13.8, 6.9   77, 34.3, 25.8, 19, 18.2, 17.5, 16.8, 15.3, 15, 13.8, 6.9  

Antibody dilution

 1:250   1:270   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA041344

  

Antibody HPA049132

  

Antibody CAB000362

  

Antibody CAB005419

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:500   1:2200      

ANTIGEN INFORMATION
 
 

Antibody HPA041344

  

Antibody HPA049132

  

Antibody CAB000362

  

Antibody CAB005419

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known   Recombinant protein  

Length (aa)

 41   36      

Antigen sequence

 
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQ
  
GMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEG
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					UBC-001 - ENSP00000344818 [100%] 
UBC-003 - ENSP00000443053 [100%] 
UBC-007 - ENSP00000441543 [100%] 
UBC-008 - ENSP00000441556 [100%] 
UBC-009 - ENSP00000442800 [100%] 
UBC-011 - ENSP00000441238 [100%] 
UBC-012 - ENSP00000438289 [100%] 
UBC-013 - ENSP00000437452 [100%] 
UBC-014 - ENSP00000445337 [100%] 
UBC-015 - ENSP00000439492 [100%] 
UBC-016 - ENSP00000440205 [100%] 
UBC-201 - ENSP00000438394 [100%] 

											 
																						UBC-001 - ENSP00000344818 [100%] 
UBC-003 - ENSP00000443053 [100%] 
UBC-007 - ENSP00000441543 [100%] 
UBC-009 - ENSP00000442800 [100%] 
UBC-011 - ENSP00000441238 [100%] 
UBC-012 - ENSP00000438289 [100%] 
UBC-013 - ENSP00000437452 [100%] 
UBC-014 - ENSP00000445337 [100%] 
UBC-015 - ENSP00000439492 [100%] 
UBC-016 - ENSP00000440205 [100%] 
UBC-201 - ENSP00000438394 [100%] 
UBC-008 - ENSP00000441556 [97%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					RPS27A - ENSG00000143947 [100%]
UBB - ENSG00000170315 [100%]
UBA52 - ENSG00000221983 [100%]

											 
																						RPS27A - ENSG00000143947 [97%]
UBB - ENSG00000170315 [100%]
UBA52 - ENSG00000221983 [97%]

											 
																						
											 
																						
											 
																				

																		

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UBC-001
UBC-003
UBC-007
UBC-008
UBC-009
UBC-011
UBC-012
UBC-013
UBC-014
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UBC-016
UBC-201
								

							

						

							
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