CDH2 - Antibodies - The Human Protein Atlas

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CDH2

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA058574

Antibody CAB000141

Antibody CAB018580

Provider

Atlas Antibodies
Sigma-Aldrich

Zymed

Santa Cruz Biotechnology

Product name

HPA058574

18-0224

sc-8424

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Not known

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA058574

Antibody CAB000141

Antibody CAB018580

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA058574

Antibody CAB000141

Antibody CAB018580

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cell junctions.

Antibody dilution

1:154

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA058574

Antibody CAB000141

Antibody CAB018580

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human heart muscle shows distinct positivity in intercalated disc.

Immunohistochemical staining of human liver shows strong cytoplasmic and membranous positivity in hepatocytes.

Immunohistochemical staining of human heart muscle shows strong membranous positivity in myocytes.

Expression

RNA: detected in 28 tissues
Protein: detected in 31 cell types

RNA: detected in 28 tissues
Protein: detected in 27 cell types

RNA: detected in 28 tissues
Protein: detected in 39 cell types

Retrieval

HIER pH6

Antibody dilution

1:20

1:150

1:40

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA058574

Antibody CAB000141

Antibody CAB018580

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

99.8, 97

99.8, 97, 21.9, 13.8, 8.5

Antibody dilution

1:80

1:500

PROTEIN ARRAY

Antibody HPA058574

Antibody CAB000141

Antibody CAB018580

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:650

ANTIGEN INFORMATION

Antibody HPA058574

Antibody CAB000141

Antibody CAB018580

Antigen

Recombinant protein fragment

Not known

Length (aa)

Antigen sequence

NSNDGLVTVVKPIDFETNRMFVLTVAAENQVPLAKGIQHPPQSTATVSVT
VIDVNENPYFAPNPKIIRQEEGLHAGTMLTTFTAQDP

Matching transcripts

CDH2-001 - ENSP00000269141 [100%]
CDH2-003 - ENSP00000382312 [100%]

Other gene match

ANTIGEN VIEW

CDH2-001	CDH2-003

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.