ARMC10 - Antibodies - The Human Protein Atlas

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ARMC10

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA011036

Antibody HPA011057

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA011036

HPA011057

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA011036

Antibody HPA011057

Immunocytochemistry

Immunohistochemistry

Supported

Western blot

Supported

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA011036

Antibody HPA011057

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA011057.

Validated

Antibody staining overlaps with antibody HPA011036.

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.

Antibody dilution

1:95

1:182

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA011036

Antibody HPA011057

Standard validation

Supported

Figure description

Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 71 cell types

RNA: detected in 37 tissues
Protein: detected in 63 cell types

Retrieval

HIER pH6

Antibody dilution

1:25

1:250

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA011036

Antibody HPA011057

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

37.5, 33.8, 31.2, 31.1, 27.4, 25.4, 24.8

37.5, 33.8, 31.2, 31.1, 27.4, 24.8, 20.6

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA011036

Antibody HPA011057

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA011036

Antibody HPA011057

Antigen

Recombinant protein fragment

Length (aa)

105

Antigen sequence

LTDGSYDDVLNAEQLQKLLYLLESTEDPVIIERALITLGNNAAFSVNQAI
IRELGGIPIVANKINHSNQSIKEKALNALNNLSVNVEN

LKLLLNLSENPAMTEGLLRAQVDSSFLSLYDSHVAKEILLRVLTLFQNIK
NCLKIEGHLAVQPTFTEGSLFFLLHGEECAQKIRALVDHHDAEVKEKVVT
IIPKI

Matching transcripts

ARMC10-001 - ENSP00000319412 [100%]
ARMC10-002 - ENSP00000396654 [100%]
ARMC10-003 - ENSP00000413619 [100%]
ARMC10-004 - ENSP00000405612 [100%]
ARMC10-201 - ENSP00000397969 [100%]
ARMC10-202 - ENSP00000440463 [100%]
ARMC10-007 - ENSP00000398201 [89%]

ARMC10-001 - ENSP00000319412 [100%]
ARMC10-002 - ENSP00000396654 [100%]
ARMC10-003 - ENSP00000413619 [100%]
ARMC10-004 - ENSP00000405612 [100%]
ARMC10-202 - ENSP00000440463 [87%]
ARMC10-008 - ENSP00000406840 [80%]
ARMC10-201 - ENSP00000397969 [80%]

Other gene match

ANTIGEN VIEW

ARMC10-001	ARMC10-002	ARMC10-003	ARMC10-004	ARMC10-007	ARMC10-008	ARMC10-201	ARMC10-202

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.