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Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA028285 across 75 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA019725 across 75 cell types.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human adrenal gland shows nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows nuclear and cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 34 tissues Protein: detected in 40 cell types
RNA: detected in 34 tissues Protein: detected in 48 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:60
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA019725
Antibody HPA028285
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY411756)
Target mass (kDa)
34.1
34.1, 15.5
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA019725
Antibody HPA028285
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
