FGB - Antibodies - The Human Protein Atlas

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FGB

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA001900

Antibody HPA001901

Antibody CAB008624

Provider

Atlas Antibodies
Sigma-Aldrich

AbFrontier

Product name

HPA001900

HPA001901

LF-MA0108

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Ammonium Sulfate Precipitation

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA001900

Antibody HPA001901

Antibody CAB008624

Immunohistochemistry

Western blot

Supported

Uncertain

Protein array

Supported

IMMUNOHISTOCHEMISTRY

Antibody HPA001900

Antibody HPA001901

Antibody CAB008624

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 42 cell lines.

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA001901 across 44 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA001900 across 44 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.

Immunohistochemical staining of human placenta shows extracellular positivity in blood vessels and cytoplasmic positivity in trophoblastic cells.

Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Expression

RNA: detected in 5 tissues
Protein: detected in 37 cell types

RNA: detected in 5 tissues
Protein: detected in 18 cell types

RNA: detected in 5 tissues
Protein: detected in 40 cell types

Retrieval

HIER pH6

Antibody dilution

1:4000

1:400

1:3500

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

Not consistent with RNA expression data.

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA001900

Antibody HPA001901

Antibody CAB008624

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

55.9

55.9, 31.2

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA001900

Antibody HPA001901

Antibody CAB008624

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:1000

ANTIGEN INFORMATION

Antibody HPA001900

Antibody HPA001901

Antibody CAB008624

Antigen

Recombinant protein fragment

Native protein

Length (aa)

128

144

Antigen sequence

ERKAPDAGGCLHADPDLGVLCPTGCQLQEALLQQERPIRNSVDELNNNVE
AVSQTSSSSFQYMYLLKDLWQKRQKQVKDNENVVNEYSSELEKHQLYIDE
TVNSNIPTNLRVLRSILENLRSKIQKLE

VIQNRQDGSVDFGRKWDPYKQGFGNVATNTDGKNYCGLPGEYWLGNDKIS
QLTRMGPTELLIEMEDWKGDKVKAHYGGFTVQNEANKYQISVNKYRGTAG
NALMDGASQLMGENRTMTIHNGMFFSTYDRDNDGWLTSDPRKQC

Matching transcripts

FGB-001 - ENSP00000306099 [100%]

FGB-001 - ENSP00000306099 [100%]
FGB-010 - ENSP00000426757 [100%]

Other gene match

ANTIGEN VIEW

FGB-001	FGB-010

RELEVANT PUBLICATIONS

Antibody HPA001900

Antibody HPA001901

Antibody CAB008624

Classification of protein profiles from antibody microarrays using heat and detergent treatment
Häggmark A et al
N Biotechnol 2012;29(5):564-70

Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model
Kato BS et al
Proteome Sci 2011;9:73

Profiling post-centrifugation delay of serum and plasma with antibody bead arrays
Qundos U et al
J Proteomics 2013;95:46-54

Development of a fibrinogen-specific sandwich enzyme-linked immunosorbent assay microarray assay for distinguishing between blood plasma and serum samples
Gonzalez RM et al
Anal Biochem 2011;414(1):99-102
Application: ELISA

Profiling post-centrifugation delay of serum and plasma with antibody bead arrays
Qundos U et al
J Proteomics 2013;95:46-54

Tumour expression of bladder cancer-associated urinary proteins
Lindén M et al
BJU Int 2013;112(3):407-15

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.