ATF7IP - Antibodies - The Human Protein Atlas

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ATF7IP

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA016578

Antibody HPA023505

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA016578

HPA023505

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA016578

Antibody HPA023505

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA016578

Antibody HPA023505

Formal validation: Genetic

Validated

Significant downregulation > 25% by one siRNA.

Relative fluorescence intensity (RFI)

Figure description

Nuclear region of segmented cells in 10x-images

Antibody dilution

1:172

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA023505.

Validated

Antibody staining overlaps with antibody HPA016578.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.

Antibody dilution

1:54

1:200

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA016578

Antibody HPA023505

Standard validation

Supported

Figure description

Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.

Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 65 cell types

RNA: detected in 37 tissues
Protein: detected in 68 cell types

Retrieval

HIER pH6

Antibody dilution

1:225

1:2000

Literature conformity

Partly consistent with extensive gene/protein characterization data.

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA016578

Antibody HPA023505

Formal validation: Genetic

Validated

Downregulation visible in both siRNA lanes

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled

Target mass (kDa)

137.5, 136.4, 136.3, 118.7, 55

Loading control

Total protein image

Antibody dilution

1:383

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

137.5, 136.4, 136.3, 118.7, 55, 19, 18.9, 18.3, 16.7, 14.9, 14.3

137.5, 136.4, 136.3, 118.7, 55

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA016578

Antibody HPA023505

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA016578

Antibody HPA023505

Antigen

Recombinant protein fragment

Length (aa)

113

131

Antigen sequence

YKVKEELLKTDVKLLNGNHENGDLDPTSPLENMDYIKDKEEVNGIEEICF
DPEGSKAEWKETPCILSVNVKNKQDDDLNCEPLSPHNITPEPVSKLPAEP
VSGDPAPGDLDAG

SMEIDQGEKNEDETSADLVETINENVIEDNKSENILENTDSMETDEIIPI
LEKLAPSEDELTCFSKTSLLPIDETNPDLEEKMESSFGSPSKQESSESLP
KEAFLVLSDEEDISGEKDESEVISQNETCSP

Matching transcripts

ATF7IP-001 - ENSP00000445955 [100%]
ATF7IP-002 - ENSP00000440440 [100%]
ATF7IP-004 - ENSP00000443179 [100%]
ATF7IP-010 - ENSP00000444784 [100%]
ATF7IP-011 - ENSP00000439279 [100%]
ATF7IP-013 - ENSP00000441483 [100%]
ATF7IP-014 - ENSP00000439578 [100%]
ATF7IP-018 - ENSP00000379575 [100%]
ATF7IP-019 - ENSP00000446260 [100%]
ATF7IP-024 - ENSP00000444589 [100%]
ATF7IP-201 - ENSP00000261168 [100%]
ATF7IP-022 - ENSP00000443540 [91%]
ATF7IP-023 - ENSP00000414158 [85%]

ATF7IP-001 - ENSP00000445955 [100%]
ATF7IP-002 - ENSP00000440440 [100%]
ATF7IP-004 - ENSP00000443179 [100%]
ATF7IP-024 - ENSP00000444589 [100%]
ATF7IP-201 - ENSP00000261168 [100%]
ATF7IP-018 - ENSP00000379575 [97%]

Other gene match

ANTIGEN VIEW

ATF7IP-001	ATF7IP-002	ATF7IP-004	ATF7IP-010	ATF7IP-011	ATF7IP-013	ATF7IP-014	ATF7IP-018	ATF7IP-019	ATF7IP-022	ATF7IP-023	ATF7IP-024	ATF7IP-201

RELEVANT PUBLICATIONS

Antibody HPA016578

Antibody HPA023505

The transcriptional regulator Aire coopts the repressive ATF7ip-MBD1 complex for the induction of immunotolerance
Waterfield M et al
Nat Immunol 2014;15(3):258-65
Application: ICC-IF

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.