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SPTLC3

ANTIBODY INFORMATION
 
 

Antibody HPA044247

  

Antibody HPA048079

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA044247   HPA048079  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 12   16  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA044247

  

Antibody HPA048079

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Approved    
Western blot

Uncertain   Supported  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA044247

  

Antibody HPA048079

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line Hep G2 shows localization to microtubules.  

Antibody dilution

   1:68  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA044247

  

Antibody HPA048079

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human placenta shows strong cytoplasmic and membranous positivity in trophoblastic cells.    

Expression

 RNA: detected in 35 tissues
Protein: detected in 30 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:350    

Literature conformity

 Partly consistent with gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA044247

  

Antibody HPA048079

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 62   62, 16  

Antibody dilution

 1:250   1:340  

PROTEIN ARRAY
 
 

Antibody HPA044247

  

Antibody HPA048079

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:7000  

ANTIGEN INFORMATION
 
 

Antibody HPA044247

  

Antibody HPA048079

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 61   58  

Antigen sequence

 
SYNFLGLAAKYDESMRTIKDVLEVYGTGVASTRHEMGTLDKHKELEDLVA
KFLNVEAAMVF
  
MANPGGGAVCNGKLHNHKKQSNGSQSRNCTKNGIVKEAQQNGKPHFYDKL
IVESFEEA
 

Matching transcripts

 SPTLC3-006 - ENSP00000381968 [100%]
  SPTLC3-001 - ENSP00000389749 [100%]
SPTLC3-006 - ENSP00000381968 [100%]
 

Other gene match

    
ANTIGEN VIEW
SPTLC3-001
SPTLC3-006
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.