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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.
Antibody dilution
1:63
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA064763
Antibody CAB008980
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human kidney shows moderate cytoplasmic and nuclear positivity in cells in tubules while moderate nuclear staining in cells in glomeruli.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
Expression
RNA: detected in 37 tissues Protein: detected in 58 cell types
RNA: detected in 37 tissues Protein: detected in 62 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:450
1:700
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA064763
Antibody CAB008980
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Target mass (kDa)
105.1
105.1
Antibody dilution
1:210
1:500
PROTEIN ARRAY
Antibody HPA064763
Antibody CAB008980
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.