ZNF266 - Antibodies - The Human Protein Atlas

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ZNF266

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA026836

Antibody HPA054110

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA026836

HPA054110

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA026836

Antibody HPA054110

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA026836

Antibody HPA054110

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus & vesicles.

Immunofluorescent staining of human cell line HeLa shows localization to rods & rings & vesicles.

Antibody dilution

1:200

1:4

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA026836

Antibody HPA054110

Standard validation

Uncertain

Figure description

Immunohistochemical staining of human tonsil shows strong cytoplasmi positivity in germinal center and non-germinal center cells.

Immunohistochemical staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 64 cell types

RNA: detected in 37 tissues
Protein: detected in 43 cell types

Retrieval

HIER pH6

Antibody dilution

1:1000

1:10

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA026836

Antibody HPA054110

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

62.1

Antibody dilution

1:250

1:80

PROTEIN ARRAY

Antibody HPA026836

Antibody HPA054110

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA026836

Antibody HPA054110

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

NLATVGYQLFKPSLISWLEQEESRTVQRGDFQASEWKVQLKTKELALQQD
VLGEPTSSGIQMIGSHNGGEVSDVKQCGDVSSEHSCLKTHV

NSENTFECYLYGVDFLTLHKKTSTGEQRSVFSQC

Matching transcripts

ZNF266-001 - ENSP00000468491 [100%]
ZNF266-002 - ENSP00000466714 [100%]
ZNF266-003 - ENSP00000467151 [100%]
ZNF266-004 - ENSP00000467403 [100%]
ZNF266-005 - ENSP00000467315 [100%]

Other gene match

ANTIGEN VIEW

ZNF266-001	ZNF266-002	ZNF266-003	ZNF266-004	ZNF266-005

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.