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CADM2

ANTIBODY INFORMATION
 
 

Antibody HPA010024

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA010024  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 4  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA010024

  
Immunohistochemistry


Validated
  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA010024

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

Cell line0100200300400500600700Protein expression
 
SH-SY5YU-138 MGU-251 MGU-87 MGA-431HaCaTSK-MEL-30WM-115HELHL-60HMC-1K-562NB-4THP-1U-937DaudiHDLM-2Karpas-707MOLT-4REHRPMI-8226U-266/70U-266/84U-698A549SCLC-21HCACO-2CAPAN-2Hep G2AN3-CAEFO-21HeLaMCF7SiHaSK-BR-3HEK 293NTERA-2PC-3RT4RH-30U-2 OSU-2197TIMEBEWO0100200300400500600700Protein expressionBrainSkinMyeloidLymphoidLungAbdominalBreast, female reproductive systemRenal, urinary, male reproductive systemSarcomaEndothelialMiscellaneous

Cell line051015202530RNA expression
 
SH-SY5YU-138 MGU-251 MGU-87 MGA-431HaCaTSK-MEL-30WM-115HELHL-60HMC-1K-562NB-4THP-1U-937DaudiHDLM-2Karpas-707MOLT-4REHRPMI-8226U-266/70U-266/84U-698A549SCLC-21HCACO-2CAPAN-2Hep G2AN3-CAEFO-21HeLaMCF7SiHaSK-BR-3HEK 293NTERA-2PC-3RT4RH-30U-2 OSU-2197TIMEBEWO051015202530RNA expressionBrainSkinMyeloidLymphoidLungAbdominalBreast, female reproductive systemRenal, urinary, male reproductive systemSarcomaEndothelialMiscellaneous

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil.  

Expression

 RNA: detected in 17 tissues
Protein: detected in 7 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:150  

Literature conformity

 Consistent with gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA010024

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

 

Figure description

  

Target mass (kDa)

 47.6  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA010024

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA010024

  

Antigen

 Recombinant protein fragment  

Length (aa)

 139  

Antigen sequence

 
EIHYTPSVKIIPSTPFPQEGQPLILTCESKGKPLPEPVLWTKDGGELPDP
DRMVVSGRELNILFLNKTDNGTYRCEATNTIGQSSAEYVLIVHDVPNTLL
PTTIIPSLTTATVTTTVAITTSPTTSATTSSIRDPNALA
 

Matching transcripts

 CADM2-001 - ENSP00000384193 [100%]
CADM2-006 - ENSP00000384575 [100%]
 

Other gene match

  
ANTIGEN VIEW
CADM2-001
CADM2-006
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.