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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in granular pattern in tubular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 56 cell types
Retrieval
HIER pH6
Antibody dilution
1:1000
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA014849
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401689)
Target mass (kDa)
11.7, 11.3, 5.8, 5.5
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA014849
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
