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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in subsets of cells in seminiferus ducts(spermatids).
Expression
RNA: detected in 1 tissues Protein: detected in 52 cell types
RNA: detected in 1 tissues Protein: detected in 44 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:140
1:65
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA038919
Antibody HPA039241
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409298)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409298)
Target mass (kDa)
27.7
27.7, 22.7
Antibody dilution
1:250
1:125
PROTEIN ARRAY
Antibody HPA038919
Antibody HPA039241
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
