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STAP2

ANTIBODY INFORMATION
 
 

Antibody HPA002375

  

Antibody HPA027761

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA002375   HPA027761  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 3   5  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA002375

  

Antibody HPA027761

  
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002375

  

Antibody HPA027761

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.   Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in subsets of cells.  

Expression

 RNA: detected in 34 tissues
Protein: detected in 68 cell types		   RNA: detected in 34 tissues
Protein: detected in 16 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:10   1:35  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002375

  

Antibody HPA027761

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 49.6, 44.9   49.6, 44.9, 29.4  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA002375

  

Antibody HPA027761

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA002375

  

Antibody HPA027761

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 101   124  

Antigen sequence

 
NYFVSHTKKALVPFLLDEDYEKVLGYVEADKENGENVWVAPSAPGPGPAP
CTGGPKPLSPASSQDKLPPLPPLPNQEENYVTPIGDGPAVDYENQDVASS
S
  
QGLTIYFYNSNRDFQHVEKLNLGAFEKLTDEIPWGSSRDPGTHFSLILRN
QEIKFKVETLECREMWKGFILTVVELRVPTDLTLLPGHLYMMSEVLAKEE
ARRALETPSCFLKVSRLEAQLLLE
 

Matching transcripts

 STAP2-002 - ENSP00000468927 [100%]
STAP2-003 - ENSP00000471052 [100%]
  STAP2-002 - ENSP00000468927 [99%]
STAP2-003 - ENSP00000471052 [99%]
STAP2-006 - ENSP00000472851 [87%]
 

Other gene match

    
ANTIGEN VIEW
STAP2-002
STAP2-003
STAP2-006
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.