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Immunofluorescent staining of human cell line PC-3 shows localization to nucleoli fibrillar center.
Antibody dilution
1:154
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA026562
Antibody HPA028260
Antibody HPA052429
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in purkinje cells.
Expression
RNA: detected in 35 tissues Protein: detected in 19 cell types
RNA: detected in 35 tissues Protein: detected in 16 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:200
1:20
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA026562
Antibody HPA028260
Antibody HPA052429
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
137.5
137.5
137.5
Antibody dilution
1:250
1:250
1:770
PROTEIN ARRAY
Antibody HPA026562
Antibody HPA028260
Antibody HPA052429
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
